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The objective of the present study was, when considering the Oxa FUFA combination, to examine the impact of one given drug on the cellular determinants of cytotoxic activity of the other drug. Different potentially relevant cellular parameters for the activity of combined drugs were analysed on the human colon cancer cell line WiDr in clinically relevant conditions of drug exposure. The tested drug sequence was Oxa FA during 2 followed by FU during 48 This sequence faithfully matches the currently applied clinical protocol derived from the Gramont schedule (De Gramont et al, 2000).

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Obtained archival human liver biopsy tissue and divided patients into groups based on their case history and disease severity. Carried out all morphological analyses of tissue sections and scoring of patient liver pathology. Carried out all in vivo experiments.

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