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(a) During standard differentiation, iPSC lines 27e and 29e showed abnormal embryoid body morphology and survival compared to lines that behaved normally (HuES 3 and 29d shown); phase scale bar, 500 DNA scale bar, 129 (b) Although embryoid bodies from iPSC line 11b had typical morphology, day 32 cultures showed decreased neuronal TUJ1 staining compared to all other normal lines (HuES 13 and iPSC 18a shown), scale bar, 129 (c) Representative phase and immunostaining images for previously defective iPSC lines 29e, 11b, and control ESC lines HuES 3 and HuES 3 hb9:GFP. Phase image scale bars are 500 immunostaining image scale bars are 100 (d Quantification of immunostaining in differentiated cultures derived from the three previously problematic iPSC lines (11b, 27e, 29e) and ESC controls; percentage of TUJ1+ cells (d); percentage of ISL+ cells (e); and percentage of HB9+ cells (f). Maintained human fibroblasts.
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